Research Associate, Cellular & Molecular Neurobiology
CODA Biotherapeutics (www.codabiotherapeutics.com) is an early-stage venture-backed biotechnology company located in South San Francisco, California. Our chemogenetics platform aims to treat neurological indications through modulation of specific target cell activity utilizing engineered ligand-gated ion channels delivered via gene therapy. The engineered ligand-gated ion channels are designed to interact with defined small molecule therapeutics and act as a switch to activate or inactivate specific neuronal populations as a means for treating neurological diseases.
We are looking for a highly motivated and dynamic candidate for the role of Research Associate, Cellular & Molecular Neurobiology to join CODA’s pharmacology group. This individual will work under the guidance of a scientist to support selection and characterization of engineered ligand-gated ion channel candidates via in vitro imaging and electrophysiology assays. The ideal candidate has significant experience in neuronal primary culture, including tissue harvest, cell culture and in vitro viral vector transduction systems, and is open-minded, trainable, and enthusiastic about research.
- Tissue Harvest: Responsible for dissection of CNS and PNS tissue from rodents, and isolation of cell-bearing areas for downstream primary culture purposes. Areas of interest include, but are not limited to, sensory neuron ganglia and hippocampus.
- Neuronal Cell Culture: Responsible for preparing, maintaining and troubleshooting one or more rodent primary neuronal subtypes in culture. Also responsible for preparing and maintaining cultures from human primary sensory neuronal tissue. Culture, maintain and troubleshoot two or more human IPSC-derived neuronal cell lines.
- In vitro Transductions: Perform transductions of neuronal cultures using AAV-based viral vectors, and oversee stocks of virus. Responsible for maintaining, transfecting, and transducing IPSC-derived and standard mammalian cell lines. Ability to design and execute experiments for culture and transduction condition optimization.
- In vitro Imaging Assays: Perform immunocytochemistry and live-cell based assays in support of engineered ligand-gated ion channel candidate characterization.
- Organization and Communication of Data: Communicate data in written and oral modalities in both informal and formal settings. Generate and manage meticulous method protocols, data, and analysis reports in a clear and highly organized fashion.
- General Lab Duties: Responsible for fulfilling company-wide drug compound requests. Responsible for making solutions, buffers, and compound dilutions to support electrophysiology and cellular & molecular neuroscience. Duties also include ordering, receiving and stocking supplies, inventory tracking, and representing group in company stockroom committee.
- S or M.S. in Cell Biology or a closely related field with at least 1-2 years of post-degree research experience in an academic or industry setting.
- Extensive experience and expertise in harvesting, isolating and culturing of primary neurons from rodents, and culturing and differentiation of human iPSCs.
- Extensive experience in mammalian cell culture, transfection and transduction, including IPSC-derived neuronal cells.
- Experience in working with viral vectors (AAV, HSV, LV).
- Experience with immunofluorescence and other imaging-based in vitro assays.
- Proficiency with common laboratory equipment (balances, pH meter, osmometer, etc.)
- Experience with common lab practices and safety.
- Good working knowledge of MS office, data plotting and graphing software (e.g. Prism) and ability to communicate results effectively.
- 2-3 years of post-degree research experience in an industry setting preferred.
- Familiarity with ion channels, GPCRs, chemogenetics, and/or optogenetics.
- Experience with gene expression constructs, basic molecular biology (e.g. PCR, cloning) and biochemistry (e.g. western blotting).
- Specific experience in quantitative analysis of imaging data.
- Experience with data processing and analysis, particularly with programming / scripting (e.g. Python, R, ImageJ).
- Dynamic self-starter with a drive for results
- Motivates others to do their best; celebrates differences; team-oriented
- Strong attention to detail
- Impeccable integrity
- Scientific curiosity paired with a strong work ethic and grit
- Thrives in a dynamic and fast-paced entrepreneurial setting and fluidly adapts to change as the company grows
- Embraces the lean start-up mentality and never says or enjoys hearing “that’s not my job”
Job Type: Full-time
Salary: commensurate with role and experience
CODA Biotherapeutics, Inc. is an Equal Opportunity Employer, and offers a comprehensive benefits package including health, dental, generous paid time off and much more.